Animal Cell Division: Colchicine and Essay

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The main side effect of colchicines on animals is nausea. The use of colchicines on animals has also generated numerous concerns regarding the toxicity of bone marrow because of the ability of these substances to interfere with cell division. Furthermore, these substances are also likely to cause urine dip stick to wrongly read positive for blood. Colchicines can not only enhance the level of alkaline phosphatase as recorded on a blood chemistry panel but also diminish the body of vitamin B-12 in certain cases.

Since cytochalasins bind actin monomers and prevents their congregation into microfilaments, the already formed microfilaments slowly depolymerize. The main effect of these substances on animal cell division is that they inhibit cytoplasmic division but do not interfere with nuclear division or DNA synthesis. As a result, these substances contribute to the accumulation of large multi-nucleate cells (Gurdon & Fairman, p. 78.).

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In addition to the probability of completely blocking adenosine-induced apoptotic body formation in cultured cells, these substances are also likely to lessen actin filaments through blocking the addition of monomer at the rapidly developing end of the polymer.

Cytochalasins also cause lack of normal adhesion between cells to an extent that they create a flattened heap. In this case, the cells in such a heap are seemingly large if they have not divided and are relatively healthy. However, some degree of adhesion is present between the animal cells in a cytochalasin conjugate because they cannot be divided with pipette. When handling cytochalasins, it's always advisable to minimize or avoid direct inhalation or body contact because they are probable teratogens and highly toxic.

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