Biology Lab Term Paper

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Temperature on Enzyme Activity

Tube 2, #4: Place the tube in an incubator (or water bath) at 37 degree centigrade. After 15 minutes, what change do you now observe?

After 15 minutes the milk became partially solid.

Tube 3, #4: Add three drops of warmed rennin and return the tube to an incubator (or water bath) at 37 degree centigrade. After 15 minutes, what change do you now observe?

After 15 minutes, the milk remains liquid with no solidifying taking place.

Table 5.1 Rennin Experiment

Tube Results Explanation

Refrigerated rennin - (tube 2) 0 Enzyme not active at low temperature.


Warmed rennin - (tube 1) ++ Enzyme effective at 37c temperature.

Boiled rennin - (tube 3) 0 Enzyme not active at high temperature,

Enzyme denatured at high temperature.

Graph: Effect of Temperature on Enzyme Activity.

Laboratory Review

What happens at the active site of an enzyme?

The active site is the region of the enzyme where the substrate binds and where the chemical reaction takes place.

2. On the basis of the active site, explain why the following conditions speed a chemical reaction.

a. More enzyme. With.....

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