PCR-DNA Experiment Abstract- Science and Lab Report

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The experiment was designed to first extract DNA, take it through the steps of amplification and detachment, and then prepare it for analysis. The question was what type of bacteria was being sequenced. The steps used were: 1) Extraction of genomic DNA, 2) Amplification of genetic region, 3) Verification, 4) Clean PCR Products, 5) Quantify DNA concentration, 6) Cycle the sequence, and, 7) Precipitate cycle-sequenced products for analysis. In this case, a virtual laboratory was used, with the user being guided to the appropriate tools and equipment (Virtual Bacterial Identification).

Results

Accession

Description

Max score

Total score

Query coverage

E value

Max ident gi|39295|Z11684.1

R.henselae 16S rRNA gene

0.0

gi|6626180|AF214556.1

Bartonella henselae 16S ribosomal RNA, partial sequence

0.0

99%

Discussion- The BLAST search displays the matching sequences in the database in descending order of the degree of the match. Most of the top scorers are either Bartonella henselae or Rochalimaea henselae. The latter is an older name for this species. Bartonella species are demanding in their nutritional requirements for culture; as a result, normal culturing techniques do not yield vigorously growing bacterial colonies.

Bartonella henselae Various species of Bartonella that are pathogenic to humans are transmitted via a vector, or directly from an animal reservoir.

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For example, B. bacilliformis via sandflies causes Oroya fever; B. quintana via body lice causes trench fever; and B. henselae via cats causes cat scratch disease (CSD). CSD typically manifests as swellings of the lymph glands, possibly with skin lesions at the site of inoculation and possibly accompanied by fever, fatigue, and other symptoms. Immunocompromised patients may be particularly susceptible and can develop a different disease, bacillary angiomatosis, as a result of infection by B. henselae or B. Quintana (Virtual Bacteria Identification).

REFERENCES

Nobel, I. "Secret of Life Discovery Turns 50." 27 Feburary 2007. BBC News. August 2010 .

"Virtual Bacterial Identification." January 2010. The Virtual Bacterial ID Lab. August 2010 .

Walker and Jones. Genes and DNA. Kingfisher, OK: Kingfisher Press, 2003.

APPENDIX A -- Genetic Map of

Search:

Query ID: lcl|19505

Accession

Description

Max score

Total score

Query coverage

E value

Max ident gi|39295|Z11684.1

R.henselae 16S rRNA gene

0.0

gi|6626180|AF214556.1

Bartonella henselae 16S ribosomal RNA, partial sequence

0.0

99%

gi|2828303|AJ223779.1

Bartonella henselae 16S rRNA gene, isolate FR96/BK38.....

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