Chromatography the Purpose of This Lab Report

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e. not mixtures) that show as green or violet and so these might not be mixtures. Black might also be a mixture of these three primary colors in varying degrees, or simply contain a high concentration of a dark dye that appears black to the human eye in mot conditions.

Procedures

Artistic Patterns

1) Draw on the center of two pieces of filter paper, using only one color on one piece and two or three colors on another.

2) Using a medicine dropper, introduce water to the center of the pieces of paper, not saturating but adding water as necessary to continue driving capillary action.

3) Observe and record what happens to the marker lines when the water encounters them.

A Colorful Race

1) Draw a soft pencil line about a half-inch from the bottom of a strip of Chromatography paper, then using different markers place three dots on this line approximately one centimeter apart and not too close to the edge of the paper, either.

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2) Pour a small amount of isopropyl alcohol into a cup -- in height, the amount should be less than a half-inch so that the alcohol level is not higher than the line on the strip when standing upright in the cup. Place the strip in the cup.

3) Cover the cup with plastic to avoid evaporation during the experiment.

4) Remove the strip before the alcohol reaches the top of the strip and mark its terminus with another soft pencil line.

5) Calculate the Rf value for each spot observed by measuring the distance of the dot from the first pencil line divided by the distance traveled by the alcohol (the second pencil line).

6) Repeat the experiment using a new strip of….....

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