The purified DNA is then prepared using a PCR like procedure that is described in detail by Innis and then can be automatically sequenced using standard methods (Hirashi). The resulting DNA sequence can then be entered into the NCBI database to sear Continue Reading...
The experiment was designed to first extract DNA, take it through the steps of amplification and detachment, and then prepare it for analysis. The question was what type of bacteria was being sequenced. The steps used were: 1) Extraction of genomic Continue Reading...
Future Applications of Forensic DNA Analytical Methods
Following the description of the structure of deoxyribonucleic acid (DNA) by Nobel Prize laureates Francis Crick and James Watson in 1953, a vast array of applications have emerged based on thi Continue Reading...
Tests are therefore conducted to examine if there is a presence of S. aureus that caused the illness and if the bacteria is or can be recognised as a potential source for food poisoning (Bennet & Lancette, 2001). One such test is known under the Continue Reading...
Transcription is a process that genetic information on the DNA copies into RNA and the DNA acts as the template for the new molecules of RNA. Transcription process begins with the DNA double helix unwinding as the hydrogen bonds holding the opposing Continue Reading...
Molecular Basis Glanzmann Thrombasthenia
An investigation of the molecular basis of Glanzmann Thrombasthenia using Polymerase Chain Reaction (PCR)
The objective of this project is to investigate the molecular basis of Glanzmann Thrombasthenia (GT) Continue Reading...
" (Volpicelli-Daley and Levey, 2003)
Prior to visualization of the molecule of interest it is necessary to "fix and section the brain tissue. Double-labeling immunofluorescence is stated to detect "localization of a protein of interest as well as th Continue Reading...
Expression Profiling of a Novel Protein
A new transcription unit was discovered while working with transfected murine BAC clones, because a novel spot appeared on a 2-dimensional protein gel. Through a process of expression subcloning from the BAC c Continue Reading...
Conclusion
Despite the depressing figures embodied in the quote introducing this thesis, that: "The overall cure rate for AML…is between 40 and 45%" (Belson, Kingsley, and Holmes, para. 6), data/information related during the next chapter, t Continue Reading...
123). In this study, Martinez-Contreras and her associates report the results of recent research that has provided additional evidence concerning the function of these proteins in precursor-messenger RNA (pre-mRNA) splicing (2007).
The splicing rep Continue Reading...